CFPSILO
Owned by0xa2eC2…5cD3c
Project Description
This project investigates the dual-bottleneck mechanism in cell-free psilocin biosynthesis. Using ODE modeling and molecular docking, we identified that yield is primarily constrained by SAM-dependent methylation (requiring a 2:1 Methionine:Trp ratio) and phosphatase-dependent dephosphorylation in bypass pathways. Optimization suggests that maintaining high PsiM activity and titrating phosphatase levels are critical for overcoming metabolic stalls at norbaeocystin and psilocybin.Borat
Research Lead
Molecule DAO
Organisation
$0.00
Experiment Cost